Recombinant protein expression

Recombinant protein production

Genebeyond provides multiple proprietary protein expression platforms to meet various demands of different unique protein. Multiple E. coli expression systems can obtain the protein in 1-2 weeks at low cost and high yield. Our yeast expression system can provide high-yield bulk eukaryotic proteins with low cost culture condition. Our mammalian cell expression systems (CHO, HEK193, Hybridoma) offer active proteins with high-quality, especially requiring posttranslational modifications for the full or better functions such as IgG, GPCRs, cell/nuclear receptors, transcription factor, etc. Flexible options of transient expression and stable expression technology are provided for different projects or different research phases. Genebeyond also provides baculovirus/insect cell expression platform.

Genebeyond also offers state-of-the-art protein purification technology including affinity chromatography (AC), IMAC, ion exchange (IEX), gel filtration (SEC), hydrophobic interaction (HIC), and combination, process integration, optimization and scale-up.

Our recombinant protein expression service includes:

  • Gene codon optimization
  • Plasmid design and construction
  • E. coli
  • Yeast
  • Baculovirus/ Insect cells
  • Mammalian cells (transient or stable expression)
  • Transient transfection and expression
  • Stable single cell clone and protein production
  • Cell-free protein production
  • Fusion protein
  • Difficult protein expression