Antibody Expression and Purification
Genebeyond® monoclonal antibody (mAb) production services include:
- Monoclonal antibody (mAb) and bispecific antobody (BsAb) design
- Plasmid and expression vector design
- Multiple antibody expression platforms (E. coli, yeast, mammalian cells, hybridoma)
- Animal cell culture and fermentation process development
- Antibody purification process development and scale-up
- Antibody analysis and functional characterization
- Nonnatural amino acid-containing antibody design and production
- Fc fusion protein design and production
Genebeyond® provides multiple proprietary antibody expression platforms to meet various demands of different unique antibody. Multiple E. coli expression systems can obtain the protein in 1-2 weeks at low cost and high yield. Our yeast expression system can provide high-yield bulk eukaryotic proteins with low cost culture condition. Our mammalian cell expression systems (CHO, HEK293, Hybridoma) offer antibody with high-quality with full or better functions. Flexible options of transient expression and stable expression technology are provided for different projects or different research phases.
Genebeyond also offers state-of-the-art antibody purification technology including affinity chromatography (AC), IMAC, ion exchange (IEX), gel filtration (SEC), hydrophobic interaction (HIC), and combination, process integration, optimization and scale-up.
1. Recombinant antibody expression
- Gene codon optimization
- Plasmid design and construction
- E. coli expression system
- Yeast expression system
- Baculovirus/ insect cell expression system
- Mammalian cells (transient or stable expressions)
- Stable single cell clone and protein production
- Fc fusion proteins
2. Monoclonal antibody (mAb) process development
- Single cell line cloning
- Fed-batch cell culture process optimization
- Antibody purification (Protein A, G, L affinity chromatography, IEX, SEC, HIC, HPLC)
- Upstream and downstream process integration and scale up