Antibody Expression and Purification

Genebeyond® monoclonal antibody (mAb) production services include:

  • Monoclonal antibody (mAb) and bispecific antobody (BsAb) design
  • Plasmid and expression vector design
  • Multiple antibody expression platforms (E. coli, yeast, mammalian cells, hybridoma)
  • Animal cell culture and fermentation process development
  • Antibody purification process development and scale-up
  • Antibody analysis and functional characterization
  • Nonnatural amino acid-containing antibody design and production
  • Fc fusion protein design and production

Genebeyond® provides multiple proprietary antibody expression platforms to meet various demands of different unique antibody. Multiple E. coli expression systems can obtain the protein in 1-2 weeks at low cost and high yield. Our yeast expression system can provide high-yield bulk eukaryotic proteins with low cost culture condition. Our mammalian cell expression systems (CHO, HEK293, Hybridoma) offer antibody with high-quality with full or better functions. Flexible options of transient expression and stable expression technology are provided for different projects or different research phases.

Genebeyond also offers state-of-the-art antibody purification technology including affinity chromatography (AC), IMAC, ion exchange (IEX), gel filtration (SEC), hydrophobic interaction (HIC), and combination, process integration, optimization and scale-up.

1. Recombinant antibody expression

  • Gene codon optimization
  • Plasmid design and construction
  • E. coli expression system
  • Yeast expression system
  • Baculovirus/ insect cell expression system
  • Mammalian cells (transient or stable expressions)
  • Stable single cell clone and protein production
  • Fc fusion proteins

2. Monoclonal antibody (mAb) process development

  • Single cell line cloning
  • Fed-batch cell culture process optimization
  • Antibody purification (Protein A, G, L affinity chromatography, IEX, SEC, HIC, HPLC)
  • Upstream and downstream process integration and scale up